Conditions

Sample time points  (Minutes)

Paper reference

heat shock

[5 10 15 20 20 40 60 80]

Gasch AP, Spellman PT, Kao CM, Carmel-Harel O, Eisen MB, Storz G, Botstein D, Brown PO.

Genomic expression programs in the response of yeast cells to environmental changes.

Mol Biol Cell. 2000 Dec;11(12):4241-57

1.5 mM diamide

[5 10 20 30 40 50 60 90]

DTT 1

[5 15 30 45 60 90 120 180]

Elutriation

[0 30 60 90 120 150 180 210 240 270 300 330 360 390]

0.32 mM H2O2

[10 20 30 50 60 80 100 120 160]

DTT 2

[0 15 30 60 120 240 480]

Alpha factor

[0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 119]

Spellman PT, Sherlock G, Zhang MQ, Iyer VR, Anders K, Eisen MB, Brown PO, Botstein D, Futcher B.

Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization.

Mol Biol Cell. 1998 Dec; 9(12): 3273-3297.

1 mM MD

[10 20 30 40 50 80 105 120 160]

CDC15

[10 30 50 70 80 90 100 110 120 130 140 150 160 160 170 180 190 200 210 220 230 240 250 270 290]

CDC28

[0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160]

alpha factor, fkh1, fkh2

[0 15 30 45 60 75 90 105 120 135 150 165 180]

Zhu et al

Two yeast forkhead genes regulate the cell cycle and pseudohyphal growth.

Nature. 2000 Jul 6;406(6791):90-4.

.24 mM H2O2

[0 14 20 20 30 40 49 55 63 63 70 77 85 105 112 120 140 155 170 190 230 250 275]

Shapira M, Segal E, Botstein D.

Disruption of yeast forkhead-associated cell cycle transcription by oxidative stress.
Mol Biol Cell. 2004 Dec;15(12):5659-69. Epub 2004 Sep 15. Erratum in: Mol Biol Cell. 2005 Feb;16(2):1011.

.24 mM H2O2 (repeat)

[0 7 14 21 28 35 42 49 56 63 70 77 100 120 140 160 180 ]

2mM MD

[0 14 20 30 40 55 70 77 85 100 120 140 170 210]

0.02% MMS

[5 15 30 45 60 90 120]

 Gasch AP, Huang M, Metzner S, Botstein D, Elledge SJ, Brown PO. 

Genomic expression responses to DNA-damaging agents and the regulatory role of the yeast ATR homolog Mec1p.

Mol Biol Cell. 2001 Oct;12(10):2987-3003

mec1 plus gamma

[5 10 20 30 45 60 90 120]

wt plus gamma

[5 10 20 30 45 60 90 120]